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To demonstrate that digestarom enhance enzyme activity, a trial was conducted comparing results using a low and high digestibility diet. The results of this trial indicates that it is possible to optimizes performance by the ability to include low digestibility components. Even with a reduction of fat, the outcome in the Group fed with the low digestible diet was comparable with that achieved using the high digestibility diet.
 - Source: Manfred Kirchgessner, Tierernährung, DLG-Verlag, 10. Auflage, Abb. 6.2-3, S. 237
The selection of the raw materials used plays an important role in the enzymatic process. The enzymatic degradability of the diet depends upon the development of the enzymes in the body. During the transition between weaning and consumption of solid food, the enzymes in the intestinal tract of piglets changes from predominately lactase to enzymes such as pepsin, trypsin, amylase, maltase, and lipase. Remarkably, during the first three to four weeks of age, the secretion of hydrochloric acid in the digestive tract is very low and does not reach a normal level before seven to ten weeks of age.
It was expected that by using herbs and spices in the feedstuff, the organs of the animals in this trial would be stimulated to produce more enzymes, earlier, that are necessary to digest proteins. As a parameter for verification, the feed conversion rate and the growth rate, during the different stages of age, was used.
 - Source: Bunge Meat, Australia, 2001
The highly digestible protein sources such as meat and bone meal, blood plasma, and fishmeal were replaced by low digestibility components such as rapeseed, lupins, and legumes. With the low digestibility protein sources, the amount of non protein nitrogen (NPN), compared to total nitrogen (N), was much higher than with the highly digestible protein sources.
 - Source: Bunge Meat, Australia, 2001
Within the first seven days both groups fed with digestarom, Group B and Group D, had a higher feed intake, a higher growth rate, and a better feed conversion rate than Group A and Group C.
 - Source: Bunge Meat, Australia, 2001
Between the 26th to 33rd day of age, piglets normally do not have enough enzyme production. However, it was possible to optimise performance by using digestarom in Group D, as well as in Group B. Besides, Group C had a significantly lower growth rate and a lower feed intake. This is a sign of a lower passage rate between the stomach and the intestine as more time is needed for the enzymatic reduction of the diet.
The chemo sensors in the duodenum control the passage rate in adjusting the feed intake, for example, by a lack of nitrogen division, and they reduce in this case, stomach peristaltic.
These circumstances suggest the reasons for the low results in Group C. The question is, why did these phenomena not occur in Group D? One hypothesis is that with the change in the protein sources, the amount of NPN, in total N, has increased. It was possible to verified this hypothesis in the broiler trial, conducted at the University of Vilnius, Lithuania.
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